Chromatography
Planning B- This experiment is an analysis of the separation of plant pigments. The analysis is with with(p) to examine the types of pigments found in spinach plant leaves mixed with acetone. The stems of the spinach were removed. The remaining leaves were squashed to overhearher in an acetone-filled mortar victimisation a pestle. After obtaining a green mush, chromatography was ready to be done. Three pieces of chromatography paper were pass over to a similar number to the long pointed centrifugate tube. A capillary tube was cut at the end to absorb some of the green pigment. using the tube, a head of green was dabbed onto the different papers 1 cm above the tip and dipped into a solvent-filled centrifuge tube. Each paper was dipped into one of three solvents. The unsettled was controlled by making sure that each dot was the aim same green so there was an equal opthalmic view of the results. After 15-20 mins the tube was checked. The distance from the sunrise(prenominal) dot to the original dot was measured in centimeters. The various new colors that appeared were taken note of and compared.
Limiting Factors
Planning A- The purpose of this experiment was to identify the presence of starch in two different types of leaves through two types of methods. Two limiting factors, chlorophyll and light, were observed.
Qualitative measuring stick was done to measure how much starch, if any at all, was produced and how the flip-flop reacted to light.
Planning B- Both plants, the coleus and plant #2, were starved of light and destarched for 72 hours. After being starved, a stencil was applied to a leaf from plant #2 which was then attached to a lamp. All parts of the leaf except the stencil were cover with aluminum foil to control the variable of light.
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